Review





Similar Products

plasmid psp72  (Promega)
90
Promega plasmid psp72
Plasmid Psp72, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+psp72/pmc10945814__pnas__2321722121__sapp-107-0-9?v=Promega
Average 90 stars, based on 1 article reviews
plasmid psp72 - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
control psp72 plasmid  (Promega)
90
Promega control psp72 plasmid
Control Psp72 Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+psp72/pm39868815-231-34-42?v=Promega
Average 90 stars, based on 1 article reviews
control psp72 plasmid - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
ef1 alpha promoter  (Addgene inc)
93
Addgene inc ef1 alpha promoter
Ef1 Alpha Promoter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+psp72/pmc11503386-33-28-31?v=Addgene+inc
Average 93 stars, based on 1 article reviews
ef1 alpha promoter - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier
ef1alpha promoter  (Addgene inc)
93
Addgene inc ef1alpha promoter
A) Quantification of BORC KD TDP-43 immunofluorescence microscopy. Both BORC genes tested (S2 and S6) show significant decreases in untagged TDP-43 levels compared to a NT guide, indicated by blue dots. N=4 wells per genotype, 9 images per well (small gray dots). Significant p-values indicated on graph. B) Representative images of TDP-43 immunofluorescence imaging with BORCS6 KD. sgRNA plasmids contain a cytoplasmic BFP, enabling identification of cells expressing the sgRNA. Scale bar represents 20 µm. C) Schematic of pLentiCRISPR plasmid. pLentiCRISPR enables lentiviral delivery of a plasmid expressing an sgRNA of interest under a U6 promoter and Cas9 under an <t>Ef1alpha</t> promoter. This enables knockout of a gene of interest targeted by an sgRNA. D) Representative images of BORCS7 KO TDP-43 IF with lysosomes stained with anti-H4A3 antibody. Scale bar is 20 µm. E) Quantification of TDP-43 immunofluorescence on BORCS7 knockout neurons. Both BORCS7 guides significantly reduced the amount of TDP-43 IF signal compared to a NT guide, light blue dots. N=10 wells, 9 images per well (light gray dots). Significant p-values indicated on graph.
Ef1alpha Promoter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+psp72/bio_rxiv__2024__09__30__615241-277-16-26?v=Addgene+inc
Average 93 stars, based on 1 article reviews
ef1alpha promoter - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier
psp72 adml plasmid  (Addgene inc)
92
Addgene inc psp72 adml plasmid
A) Quantification of BORC KD TDP-43 immunofluorescence microscopy. Both BORC genes tested (S2 and S6) show significant decreases in untagged TDP-43 levels compared to a NT guide, indicated by blue dots. N=4 wells per genotype, 9 images per well (small gray dots). Significant p-values indicated on graph. B) Representative images of TDP-43 immunofluorescence imaging with BORCS6 KD. sgRNA plasmids contain a cytoplasmic BFP, enabling identification of cells expressing the sgRNA. Scale bar represents 20 µm. C) Schematic of pLentiCRISPR plasmid. pLentiCRISPR enables lentiviral delivery of a plasmid expressing an sgRNA of interest under a U6 promoter and Cas9 under an <t>Ef1alpha</t> promoter. This enables knockout of a gene of interest targeted by an sgRNA. D) Representative images of BORCS7 KO TDP-43 IF with lysosomes stained with anti-H4A3 antibody. Scale bar is 20 µm. E) Quantification of TDP-43 immunofluorescence on BORCS7 knockout neurons. Both BORCS7 guides significantly reduced the amount of TDP-43 IF signal compared to a NT guide, light blue dots. N=10 wells, 9 images per well (light gray dots). Significant p-values indicated on graph.
Psp72 Adml Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+psp72/pm38521065-324-1-3?v=Addgene+inc
Average 92 stars, based on 1 article reviews
psp72 adml plasmid - by Bioz Stars, 2026-07
92/100 stars
  Buy from Supplier
plasmid psp72-a-bla-a-lmjter  (GenOne Media Group)
90
GenOne Media Group plasmid psp72-a-bla-a-lmjter
A) Quantification of BORC KD TDP-43 immunofluorescence microscopy. Both BORC genes tested (S2 and S6) show significant decreases in untagged TDP-43 levels compared to a NT guide, indicated by blue dots. N=4 wells per genotype, 9 images per well (small gray dots). Significant p-values indicated on graph. B) Representative images of TDP-43 immunofluorescence imaging with BORCS6 KD. sgRNA plasmids contain a cytoplasmic BFP, enabling identification of cells expressing the sgRNA. Scale bar represents 20 µm. C) Schematic of pLentiCRISPR plasmid. pLentiCRISPR enables lentiviral delivery of a plasmid expressing an sgRNA of interest under a U6 promoter and Cas9 under an <t>Ef1alpha</t> promoter. This enables knockout of a gene of interest targeted by an sgRNA. D) Representative images of BORCS7 KO TDP-43 IF with lysosomes stained with anti-H4A3 antibody. Scale bar is 20 µm. E) Quantification of TDP-43 immunofluorescence on BORCS7 knockout neurons. Both BORCS7 guides significantly reduced the amount of TDP-43 IF signal compared to a NT guide, light blue dots. N=10 wells, 9 images per well (light gray dots). Significant p-values indicated on graph.
Plasmid Psp72 A Bla A Lmjter, supplied by GenOne Media Group, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+psp72/bio_rxiv__2023__11__10__566567-215-8-12?v=GenOne+Media+Group
Average 90 stars, based on 1 article reviews
plasmid psp72-a-bla-a-lmjter - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier

Image Search Results


A) Quantification of BORC KD TDP-43 immunofluorescence microscopy. Both BORC genes tested (S2 and S6) show significant decreases in untagged TDP-43 levels compared to a NT guide, indicated by blue dots. N=4 wells per genotype, 9 images per well (small gray dots). Significant p-values indicated on graph. B) Representative images of TDP-43 immunofluorescence imaging with BORCS6 KD. sgRNA plasmids contain a cytoplasmic BFP, enabling identification of cells expressing the sgRNA. Scale bar represents 20 µm. C) Schematic of pLentiCRISPR plasmid. pLentiCRISPR enables lentiviral delivery of a plasmid expressing an sgRNA of interest under a U6 promoter and Cas9 under an Ef1alpha promoter. This enables knockout of a gene of interest targeted by an sgRNA. D) Representative images of BORCS7 KO TDP-43 IF with lysosomes stained with anti-H4A3 antibody. Scale bar is 20 µm. E) Quantification of TDP-43 immunofluorescence on BORCS7 knockout neurons. Both BORCS7 guides significantly reduced the amount of TDP-43 IF signal compared to a NT guide, light blue dots. N=10 wells, 9 images per well (light gray dots). Significant p-values indicated on graph.

Journal: bioRxiv

Article Title: Maintenance of neuronal TDP-43 expression requires axonal lysosome transport

doi: 10.1101/2024.09.30.615241

Figure Lengend Snippet: A) Quantification of BORC KD TDP-43 immunofluorescence microscopy. Both BORC genes tested (S2 and S6) show significant decreases in untagged TDP-43 levels compared to a NT guide, indicated by blue dots. N=4 wells per genotype, 9 images per well (small gray dots). Significant p-values indicated on graph. B) Representative images of TDP-43 immunofluorescence imaging with BORCS6 KD. sgRNA plasmids contain a cytoplasmic BFP, enabling identification of cells expressing the sgRNA. Scale bar represents 20 µm. C) Schematic of pLentiCRISPR plasmid. pLentiCRISPR enables lentiviral delivery of a plasmid expressing an sgRNA of interest under a U6 promoter and Cas9 under an Ef1alpha promoter. This enables knockout of a gene of interest targeted by an sgRNA. D) Representative images of BORCS7 KO TDP-43 IF with lysosomes stained with anti-H4A3 antibody. Scale bar is 20 µm. E) Quantification of TDP-43 immunofluorescence on BORCS7 knockout neurons. Both BORCS7 guides significantly reduced the amount of TDP-43 IF signal compared to a NT guide, light blue dots. N=10 wells, 9 images per well (light gray dots). Significant p-values indicated on graph.

Article Snippet: TDP-43 coding sequence and 3’UTR were cloned into a plasmid expressing 24 SunTag repeats under an Ef1alpha promoter followed by 24 PP7 stem loops (modified from Addgene 74928).

Techniques: Immunofluorescence, Microscopy, Imaging, Expressing, Plasmid Preparation, Knock-Out, Staining